polyclonal rabbit anti keratin 1  (Abcam)

Journal: PLoS ONE

Article Title: p300 Alters Keratinocyte Cell Growth and Differentiation through Regulation of p21 Waf1/CIP1

doi: 10.1371/journal.pone.0008369

Figure Lengend Snippet: ( A ) H&E staining of organotypic raft cultures of control (scr) and p300 knock down (shp300A) cells (upper panel). Immunostaining of raft sections indicates that level of early marker keratin 1 (K1) and late marker filaggrin (Fil) are significantly reduced in knockdown cells (middle panel). Ki67 immunostaining reveals that there is an increase in the number of proliferative cells in the basal layer of p300 knockdown rafts compared to control (lower panel). ( B ) Graph represents percentage of Ki67 positive cells in p300 depleted cells, expressed relative to scrambled control (Mean +/− SE, two independent biological replicates).

Article Snippet: The following antibodies were used in this study: mouse monoclonal BrdU (BD Pharmingen 1∶100), mouse monoclonal anti-filaggrin (Neomarkers 1∶50 no retrieval), rabbit polyclonal anti-ki67 (Santa Cruz Biotechnology 1∶100), rabbit polyclonal anti-keratin 1 (Covance 1∶2500 no retrieval), and goat anti-mouse and anti-rabbit secondary conjugated to Alexafluor 488 nm or 594 nm (Molecular Probes 1∶400).

Techniques: Staining, Immunostaining, Marker

Journal: Scientific Reports

Article Title: Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images

doi: 10.1038/s41598-018-38232-9

Figure Lengend Snippet: BMMA plastic sections can be immuno-labelled and imaged using multiple imaging modalities, such as non-linear optics. ( A ) Histological stains hematoxylin and eosin can be used to outline basic cell and tissue structures, such as muscle and hair follicles of the eyelid. ( B ) Immunofluorescence staining of proteins (keratin 1 – red) and second-harmonic imaging of collagen fibers (white) enables probing of protein expression and extra-cellular matrix structure, with cell nuclei distribution identified by DAPI staining (blue). ( C ) Endogenously expressed fluorescent proteins, such as the histone H2B-GFP fusion protein in the H2B-GFP/K5tTA mouse, were imaged for fluorescence intensity analysis of GFP and co-localization with antibody stained proteins, such as keratin 5 (red). ( D ) BMMA plastic sections cut at 100 nm were contrasted with heavy metal staining (uranyl acetate and phospho-tungstic acid) and imaged using a JOEL 1010 transmission electron microscope. Differentiated Meibomian gland cells undergoing lipogenesis and holocrine secretion are readily visible. ( E ) Darkfield micrograph of a BMMA section of calcified mouse femur. (Scale bars: A–C 100 µm; D 2 µm; E 100 µm).

Article Snippet: Primary antibodies were used at a 1/1000 concentration and included mouse anti-αSMA (Sigma Aldrich – a2547), monoclonal rabbit anti- keratin 5 (Abcam ab52635), keratin 6 (Abcam ab93279) and keratin 8 (Abcam ab53280), polyclonal rabbit anti- keratin 1 (Abcam ab185628), and Ki67 (Abcam ab15580).

Techniques: Imaging, Immunofluorescence, Staining, Expressing, Fluorescence, Transmission Assay, Microscopy